The objectives of this study were to compare oestrous synchronization expression and conception rate following timed artificial insemination (TAI) with frozen-thawed X-sexed or unsexed semen in dairy and beef cows. For this study, 227 cows (dairy, n = 130 and beef, n = 97) were assigned to a 9-day Ovsynch + controlled intravaginal drug release (CIDR) protocol. All cows were TAI using X-sexed or unsexed semen from 8 sires. Each semen type was obtained from 4 sires [2 dairy (Holstein Friesian) and 2 beef (Angus)]. Pregnancy detection was performed on Days 35, 65 and 95 following TAI by transrectal ultrasonography and hand palpation. The proportion of oestrus expression was higher in dairy (85.3%) cows compared with beef (65.0%) cows (p < .05). Overall, dairy (X-sexed, 61.9% and unsexed, 62.0%) cows had greater conception rates on Day 35 compared to beef (X-sexed, 56.0% and unsexed, 52.2%) cows (p < .05). Concurrently, on Day 95, overall conception rates in dairy (X-sexed, 41.4% and unsexed, 48.5%) cows were greater than beef (X-sexed, 38.0% and unsexed, 37.0%) cows (p < .05). Pregnancy/embryo losses between Days 35 and 65 in dairy (X-sexed, 33.3% and unsexed, 18.2%) cows and beef (X-sexed, 28.6% and unsexed, 29.2%) cows were recorded (p < .05). Dairy (X-sexed, 7.7% and unsexed, 8.3%) cows had higher incidence of pregnancy losses between Days 66 and 95 when compared to beef (X-sexed, 5.0% and unsexed, 0.0%) cows (p < .05). Oestrous expression and conception rates in dairy and beef cows were satisfactory. Advanced reproductive biotechnologies can successfully utilize gender-ablated semen in organized emerging cattle farming systems.
Keywords: cattle; oestrous synchronization; sexed semen; timed artificial insemination.
© 2023 Agricultural Research Council. Reproduction in Domestic Animals published by Wiley-VCH GmbH.