Modulation of collagen synthesis by fat-storing cells, isolated from CCl4- or vitamin A-treated rats

Dig Dis Sci. 1987 Nov;32(11):1281-9. doi: 10.1007/BF01296379.

Abstract

In an attempt to elucidate the role of fat-storing cells (FSCs) in liver fibrosis, we investigated the collagen synthesis by FSCs freshly isolated from rats treated with CCl4, with vitamin A, and from untreated rats. FSCs from CCl4-treated rats contained a small number of lipid droplets and an abundant rough endoplasmic reticulum (RER), while those from vitamin A-treated rats showed numerous large lipid droplets and scanty RER. The population doubling times of FSCs isolated from normal, CCl4-treated, and vitamin A-treated rats were 38 +/- 4.3, 24 +/- 2.5, and 48 +/- 6.3 hr, respectively. The rate of collagen synthesis by FSCs from CCl4-treated rats was four- to sixfold enhanced, while collagen synthesis by FSCs from vitamin A-treated rats was suppressed. The ratio of collagen type I to type III produced by FSCs from CCl4 rats was enhanced as compared with control rats (94.7:5.3 vs 87.6:12.4). Therefore, FSCs can be considered to play an important role in the pathogenesis of liver fibrosis.

MeSH terms

  • Animals
  • Carbon Tetrachloride / adverse effects*
  • Cells, Cultured
  • Collagen / biosynthesis*
  • Diterpenes
  • Endoplasmic Reticulum / metabolism
  • Lipid Metabolism*
  • Liver / metabolism*
  • Liver / ultrastructure
  • Liver Cirrhosis, Experimental / chemically induced
  • Liver Cirrhosis, Experimental / metabolism
  • Male
  • Rats
  • Rats, Inbred Strains
  • Retinyl Esters
  • Vitamin A / analogs & derivatives*
  • Vitamin A / pharmacology

Substances

  • Diterpenes
  • Retinyl Esters
  • Vitamin A
  • retinol palmitate
  • Collagen
  • Carbon Tetrachloride