Exploring the forensic effectiveness and population genetic differentiation by self-constructed 41 multi-InDel panel in Yunnan Zhuang group

Shuyan Mei; Shaohua Yi; Meiming Cai; Yunying Zhang; Wei Cui; Hui Xu; Qiong Lan; Bofeng Zhu

doi:10.1016/j.gene.2023.147180

Exploring the forensic effectiveness and population genetic differentiation by self-constructed 41 multi-InDel panel in Yunnan Zhuang group

Gene. 2023 Apr 15:860:147180. doi: 10.1016/j.gene.2023.147180. Epub 2023 Jan 18.

Authors

Shuyan Mei¹, Shaohua Yi², Meiming Cai¹, Yunying Zhang¹, Wei Cui³, Hui Xu¹, Qiong Lan⁴, Bofeng Zhu⁵

Affiliations

¹ Guangzhou Key Laboratory of Forensic Multi-Omics for Precision Identification, School of Forensic Medicine; Microbiome Medicine Center, Department of Laboratory Medicine, Zhujiang Hospital; Southern Medical University, Guangzhou 510515, China.
² Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430074, China.
³ Guangzhou Key Laboratory of Forensic Multi-Omics for Precision Identification, School of Forensic Medicine; Microbiome Medicine Center, Department of Laboratory Medicine, Zhujiang Hospital; Southern Medical University, Guangzhou 510515, China; Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an 710004, China.
⁴ Guangzhou Key Laboratory of Forensic Multi-Omics for Precision Identification, School of Forensic Medicine; Microbiome Medicine Center, Department of Laboratory Medicine, Zhujiang Hospital; Southern Medical University, Guangzhou 510515, China. Electronic address: 18330176079@163.com.
⁵ Guangzhou Key Laboratory of Forensic Multi-Omics for Precision Identification, School of Forensic Medicine; Microbiome Medicine Center, Department of Laboratory Medicine, Zhujiang Hospital; Southern Medical University, Guangzhou 510515, China. Electronic address: zhubofeng7372@126.com.

PMID: 36669579
DOI: 10.1016/j.gene.2023.147180

Abstract

Yunnan is one of the main residences of the Zhuang group which is one of the 55 ethnic minorities in China. At present, there are relatively few researches on population genetics and forensic science of the Yunnan Zhuang group. Therefore, this study used a self-constructed panel containing 41 multi-InDel markers to analyze the genetic polymorphisms of 173 individuals from Yunnan Zhuang group. The results indicated that these 41 multi-InDels in Yunnan Zhuang group were highly polymorphic markers expect for three markers. The cumulative match probability and combined exclusion probability values of the 40 multi-InDels (MI38 marker was excluded) were 8.0671E-26 and 0.9999995959, respectively. In addition, population genetic analyses were performed on genotyping data of 41 multi-InDel markers among the Yunnan Zhuang and 26 reference populations, revealing that the Yunnan Zhuang group was genetically close to the five populations in East Asia. According to the STRUCTURE analysis, the Yunnan Zhuang group presented similar ancestral compositions to the five populations from East Asia, and when the K value was three, the five intercontinental populations showed their different genetic structures. In conclusion, the 41 multi-InDel markers could be used as an effective tool for individual identification and paternity testing of the Zhuang group in Yunnan province, as well as for their ancestry information inference studies.

Keywords: Genetic polymorphism; Multi-InDel; Population genetics; Zhuang group.

MeSH terms

Asian People* / genetics
China
Forensic Genetics / methods
Gene Frequency
Genetic Drift
Genetics, Population*
Humans
INDEL Mutation
Polymorphism, Genetic