The complete genome sequence of Pseudomonas chengduensis BC1815 for genome mining of PET degrading enzymes

Zhengguang Shi; Xue Yu; Jingjing Duan; Wenbin Guo

doi:10.1016/j.margen.2022.101008

The complete genome sequence of Pseudomonas chengduensis BC1815 for genome mining of PET degrading enzymes

Mar Genomics. 2023 Feb:67:101008. doi: 10.1016/j.margen.2022.101008. Epub 2022 Dec 14.

Authors

Zhengguang Shi¹, Xue Yu¹, Jingjing Duan², Wenbin Guo³

Affiliations

¹ School of Advanced Manufacturing, Fuzhou University, Jinjiang 362251, China; Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, Fujian, China.
² College of Environment and Ecology, Xiamen University, Xiamen 361005, Fujian, China.
³ School of Advanced Manufacturing, Fuzhou University, Jinjiang 362251, China; Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, Fujian, China. Electronic address: guo5721@tio.org.cn.

PMID: 36682853
DOI: 10.1016/j.margen.2022.101008

Abstract

Pseudomonas chengduensis BC1815, isolated from a marine sediment sample of the Pacific Ocean, can grow in mineral medium with PET plastic as sole carbon source. Here, we present the complete genome of Pseudomonas chengduensis BC1815, which will facilitate the genome mining of PET degrading enzymes. The total length of the sequenced genome consists of 5,578,440 bases, with mean G + C content of 62.65%. A total of 5150 coding genes including 65 tRNAs and 12 rRNAs were predicted in the genome. Thirteen proteins of esterase, lipase and α/β hydrolase were taken as candidates for PET degrading enzymes, in which eight were membrane bounded and the others were secretory.

Keywords: Complete genome; Degrading enzyme; PET; Pseudomonas chengduensis.

MeSH terms

Genome, Bacterial*
Geologic Sediments
Pacific Ocean
Pseudomonas* / genetics

Supplementary concepts

Pseudomonas chengduensis