Comparative study between SpermSlow™ hyaluronan and traditional sperm selection in ICSI outcome

Zygote. 2023 Apr;31(2):180-187. doi: 10.1017/S0967199422000685. Epub 2023 Jan 23.


The role of hyaluronic acid (HA) as a 'physiologic selector' is also well recognized in vitro: it has been demonstrated that spermatozoa that bind to immobilized HA in vitro are those having completed their plasma membrane remodelling, and cytoplasmic and meiotic maturation. Sperm selection using HA has been expected to increase the implantation rate in intracytoplasmic sperm injection (ICSI) cycles. This work was designed to evaluate an alternative product for slowing sperm motility that contains HA and measures its outcomes: fertilization rate, embryo quality, and implantation and pregnancy rates. The present study found a positive drift in embryo quality that was statistically significant in the study group (SpermSlow™-ICSI) with teratozoospermia compared with PVP-ICSI in the same group. There were differences in the pregnancy rate (statistically insignificant in normozoospermia, asthenozoospermia, oligozoospermia, and teratozoospermia) in the SpermSlow-ICSI group compared with PVP-ICSI. The HA-ICSI technique in assisted reproduction technology (ART) is an important way to improve fertilization rate, embryo quality, and pregnancy rate.

Keywords: Embryo quality; HA-ICSI; ICSI; Male infertility; PVP-ICSI.

MeSH terms

  • Female
  • Humans
  • Hyaluronic Acid / metabolism
  • Male
  • Pregnancy
  • Pregnancy Rate
  • Retrospective Studies
  • Semen
  • Sperm Injections, Intracytoplasmic* / methods
  • Sperm Motility
  • Spermatozoa / physiology
  • Teratozoospermia* / metabolism


  • Hyaluronic Acid