Light-controlled scaffold- and serum-free hard palatal-derived mesenchymal stem cell aggregates for bone regeneration

Zhiwei Jiang; Na Li; Qin Shao; Danji Zhu; Yuting Feng; Yang Wang; Mengjia Yu; Lingfei Ren; Qianming Chen; Guoli Yang

doi:10.1002/btm2.10334

Light-controlled scaffold- and serum-free hard palatal-derived mesenchymal stem cell aggregates for bone regeneration

Bioeng Transl Med. 2022 May 13;8(1):e10334. doi: 10.1002/btm2.10334. eCollection 2023 Jan.

Authors

Zhiwei Jiang^{1

2

3}, Na Li^{1

2

3}, Qin Shao^{1

2

3}, Danji Zhu^{1

2

3}, Yuting Feng^{1

2

3}, Yang Wang^{1

2

3}, Mengjia Yu^{1

2

3}, Lingfei Ren^{1

2

3}, Qianming Chen^{1

2

3}, Guoli Yang^{1

2

3}

Affiliations

¹ Stomatology Hospital, School of Stomatology Zhejiang University School of Medicine Hangzhou Zhejiang China.
² Zhejiang Provincial Clinical Research Center for Oral Diseases Hangzhou Zhejiang China.
³ Key Laboratory of Oral Biomedical Research of Zhejiang Province Cancer Center of Zhejiang University Hangzhou Zhejiang China.

Abstract

Cell aggregates that mimic in vivo cell-cell interactions are promising and powerful tools for tissue engineering. This study isolated a new, easily obtained, population of mesenchymal stem cells (MSCs) from rat hard palates named hard palatal-derived mesenchymal stem cells (PMSCs). The PMSCs were positive for CD90, CD44, and CD29 and negative for CD34, CD45, and CD146. They exhibited clonogenicity, self-renewal, migration, and multipotent differentiation capacities. Furthermore, this study fabricated scaffold-free 3D aggregates using light-controlled cell sheet technology and a serum-free method. PMSC aggregates were successfully constructed with good viability. Transplantation of the PMSC aggregates and the PMSC aggregate-implant complexes significantly enhanced bone formation and implant osseointegration in vivo, respectively. This new cell resource is easy to obtain and provides an alternative strategy for tissue engineering and regenerative medicine.

Keywords: bone regeneration; cell aggregates; hard palate; light‐controlled; mesenchymal stem cells.