Retrograde transport by the microtubule-associated protein MAP 1C

Nature. 1987 Nov 12-18;330(6144):181-3. doi: 10.1038/330181a0.

Abstract

Microtubules are involved in several forms of intracellular motility, including mitosis and organelle movement. Fast axonal transport is a highly ordered form of organelle motility that operates in both the anterograde (outwards from the cell body) and retrograde (from the periphery towards the cell body) direction. Similar microtubule-associated movement is observed in non-neuronal cells, and might be involved in secretion, endocytosis and the positioning of organelles within the cell. Kinesin is a mechanochemical protein that produces force along microtubules in an anterograde direction. We recently found that the brain microtubule-associated protein MAP 1C (ref. 7) is a microtubule-activated ATPase and, like kinesin, can translocate microtubules in an in vitro assay for microtubule-associated motility. MAP 1C seemed to be related to the ciliary and flagellar ATPase, dynein, which is thought to produce force in a direction opposite to that observed for kinesin. Here we report that MAP 1C, in fact, acts in a direction opposite to kinesin, and has the properties of a retrograde translocator.

MeSH terms

  • Animals
  • Biological Transport
  • Brain / metabolism
  • Cattle
  • Chlamydomonas / metabolism
  • Chlamydomonas / ultrastructure
  • Flagella / ultrastructure
  • Microtubule-Associated Proteins / metabolism*
  • Microtubules / metabolism
  • Microtubules / ultrastructure*

Substances

  • Microtubule-Associated Proteins