Predictive modeling of concentration-dependent viscosity behavior of monoclonal antibody solutions using artificial neural networks

MAbs. 2023 Jan-Dec;15(1):2169440. doi: 10.1080/19420862.2023.2169440.


Solutions of monoclonal antibodies (mAbs) can show increased viscosity at high concentration, which can be a disadvantage during protein purification, filling, and administration. The viscosity is determined by protein-protein-interactions, which are influenced by the antibody's sequence as well as solution conditions, like pH, buffer type, or the presence of salts and other excipients. To predict viscosity, experimental parameters, like the diffusion interaction parameter (kD), or computational tools harnessing information derived from primary sequence, are often used, but a reliable predictive tool is still missing. We present a modeling approach employing artificial neural networks (ANNs) using experimental factors combined with simulation-derived parameters plus viscosity data from 27 highly concentrated (180 mg/mL) mAbs. These ANNs can be used to predict if mAbs exhibit problematic viscosity at distinct concentrations or to model viscosity-concentration-curves.

Keywords: Monoclonal antibody; artificial neural network; high concentration; predictive modeling; viscosity.

MeSH terms

  • Antibodies, Monoclonal*
  • Computer Simulation
  • Neural Networks, Computer
  • Salts*
  • Solutions
  • Viscosity


  • Antibodies, Monoclonal
  • Salts
  • Solutions

Grants and funding

The author(s) reported there is no funding associated with the work featured in this article.