Electron transfer routes in nitrate-pentavalent vanadium co-contaminated system of oligotrophic microbiology niche

Haishuang Wang; Nan Chen; Chuanping Feng; Yang Deng; Mengnan Yang; Huaming Guo

doi:10.1016/j.scitotenv.2023.161834

Electron transfer routes in nitrate-pentavalent vanadium co-contaminated system of oligotrophic microbiology niche

Sci Total Environ. 2023 Apr 20:870:161834. doi: 10.1016/j.scitotenv.2023.161834. Epub 2023 Jan 25.

Authors

Haishuang Wang¹, Nan Chen², Chuanping Feng¹, Yang Deng³, Mengnan Yang⁴, Huaming Guo¹

Affiliations

¹ School of Water Resources and Environment, MOE Key Laboratory of Groundwater Circulation and Environmental Evolution, China University of Geosciences (Beijing), Beijing 100083, China.
² School of Water Resources and Environment, MOE Key Laboratory of Groundwater Circulation and Environmental Evolution, China University of Geosciences (Beijing), Beijing 100083, China. Electronic address: chennan@cugb.edu.cn.
³ Department of Environmental Engineering, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China.
⁴ Land Resources Consolidation and Rehabilitation Center, China GEO-Engineering Corporation, Beijing 100093, China.

PMID: 36708832
DOI: 10.1016/j.scitotenv.2023.161834

Abstract

Microbial techniques have been extensively used for the remediation of nitrate and V(V) co-contaminations, but the mechanisms of electron and substances transport and metabolism of co-contaminations under oligotrophic niche have been largely overlooked. This study quantified the electron transfer and consumption, substance transfer, and metabolic pathways in the nitrate and V(V) co-contamination system under oligotrophic condition to explore the underlying mechanisms by characterizing the products and elucidating conventional cognitive pathways. This study compared the composition of the precipitates under the conditions of sufficient and insufficient carbon sources using energy-dispersive X-ray spectroscopy, X-ray diffraction and X-ray photoelectron spectroscopy, and discovered the re-oxidation process of the already reduced V(IV). Electronic evidence for the re-oxidation process of V(IV) was also provided by electron transfer and quantitative analysis. Besides, this study found that the electron contribution ratio of NO₃^--N → NO₂^--N and V(V) → V(IV) reduction was 40.2:1. In addition, based on the functional prediction of PICRUSt 2, it was found that the utilization of intracellular reserve carbon source and enzymes in the transport chain were enhanced in oligotrophic microbiology niche. These results provide new insights into the stability of co-contamination reduction in oligotrophic microbiology niche and demonstrate a new mobilization pathway for V(V) in oligotrophic systems.

Keywords: Electron transfer; Nitrate; Oligotrophic microbiology niche; Pentavalent vanadium; Substance metabolism.

MeSH terms

Electron Transport
Electrons
Nitrates*
Oxidation-Reduction
Vanadium* / metabolism

Substances

Vanadium
Nitrates