Reduced immunogenicity and endowed antimicrobial activity in β-lactoglobulin by preparing edible bioconjugate

Tadashi Yoshida; Risa Ikeda; Shinya Kawai; Kengo Horiuchi; Yuho Matsuo; Makoto Hattori

doi:10.1007/s10616-022-00558-8

Reduced immunogenicity and endowed antimicrobial activity in β-lactoglobulin by preparing edible bioconjugate

Cytotechnology. 2023 Feb;75(1):39-47. doi: 10.1007/s10616-022-00558-8. Epub 2022 Nov 17.

Authors

Tadashi Yoshida¹, Risa Ikeda¹, Shinya Kawai¹, Kengo Horiuchi¹, Yuho Matsuo¹, Makoto Hattori¹

Affiliation

¹ Department of Applied Biological Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-Cho, Fuchu, Tokyo 183-8509 Japan.

Abstract

β-lactoglobulin (BLG) and ε-polylysine (PL) were bound by using microbial transglutaminase. Dextran (Dex) was further conjugated to the BLG-PL conjugate by the Maillard reaction. Confirmation of conjugation was carried out by SDS-PAGE. From the results of isoelectric focusing, it was revealed that the isoelectric point of the BLG-PL conjugate was shifted to the basic side as compared with native BLG. Immunogenicity of BLG in BALB/c mice was lowered by conjugation with PL and further lowered by conjugation with Dex. By conjugation with PL and PL-Dex, antibacterial activity against Staphylococcus aureus was endowed to BLG. Because the conjugation method in this study is a safe method, it is valuable in that it can be applicable to food processing.

Keywords: Functional improvement; Neoglycoconjugate; Protein conjugation; β-Lactoglobulin.

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