Vitamin D and its metabolites in the pollen of pine. Part 5: Steroid hormones in the pollen of pine species

Pharmazie. 1987 Jul;42(7):471-2.


Unconjugated vitamin D and its metabolites were investigated in the pollen of Pinus nigra Ar. and Pinus sylvestris L. by TLC, HPLC and competitive radiochemical determination of 25-hydroxycholecalciferol (25-OHD3). It was found that vitamin D (D2, D3) was present in the pollen in amounts about 2 micrograms/10 g and 25-OHD3, 24,25-dihydroxycholecalciferol [24,25-(OH)2D3] and 1,25-dihydroxycholecalciferol [1,25-(OH)2D3] between 0.1 and 3 micrograms/10 g of pollen, dependent on pollen species and methods.

MeSH terms

  • Chromatography, High Pressure Liquid
  • Chromatography, Thin Layer
  • Plants / analysis*
  • Pollen / analysis*
  • Protein Binding
  • Steroids / analysis*
  • Vitamin D / analysis*


  • Steroids
  • Vitamin D