Impact of aerobic/anoxic alternation number on performance, microbial community and functional genes of sequencing batch biofilm reactor treating mariculture wastewater

Qianzhi Wang; Yunhao Pan; Guangyu Chu; Shuailing Lu; Zhiming Zhang; Yangguo Zhao; Chunji Jin; Mengchun Gao

doi:10.1016/j.biortech.2023.128699

Impact of aerobic/anoxic alternation number on performance, microbial community and functional genes of sequencing batch biofilm reactor treating mariculture wastewater

Bioresour Technol. 2023 Mar:372:128699. doi: 10.1016/j.biortech.2023.128699. Epub 2023 Jan 31.

Authors

Qianzhi Wang¹, Yunhao Pan², Guangyu Chu³, Shuailing Lu², Zhiming Zhang², Yangguo Zhao⁴, Chunji Jin², Mengchun Gao⁵

Affiliations

¹ Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China; Shandong Provincial Key Laboratory of Marine Environment and Geological Engineering, Ocean University of China, Qingdao 266100, China; College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
² Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China.
³ Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China; College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
⁴ Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China; Shandong Provincial Key Laboratory of Marine Environment and Geological Engineering, Ocean University of China, Qingdao 266100, China.
⁵ Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China; Shandong Provincial Key Laboratory of Marine Environment and Geological Engineering, Ocean University of China, Qingdao 266100, China. Electronic address: mengchungao@outlook.com.

PMID: 36731617
DOI: 10.1016/j.biortech.2023.128699

Abstract

The performance, microbial community and functional genes of a sequencing batch biofilm reactor (SBBR) were investigated in treating mariculture wastewater under different aerobic/anoxic alternation number. The removal efficiency of chemical oxygen demand (COD) and NH₄⁺-N kept at 95.66 ± 1.83 % and 90.28 ± 2.42 % under aerobic/anoxic alternation number between 1 and 4. The total nitrogen (TN) removal efficiency gradually decreased from 94.45 ± 1.12 % to 83.06 ± 1.25 % with the increase of aerobic/anoxic alternative number from 1 to 4. The nitrification rates and their corresponding enzymatic activities increased slightly with the increase of aerobic/anoxic alternation number, whereas the denitrifying process had the contrary results. The variation of aerobic/anoxic alternation number obviously affected the microbial diversity and abundance. The microbial network structure and keystone taxa were different under different aerobic/anoxic alternation number. The functional genes abundance for the denitrification pathway decreased with the increase of aerobic/anoxic alternation number.

Keywords: Aerobic/anoxic alternation number; Functional gene; Mariculture wastewater; Nitrogen removal rate; Tax4Fun analysis.

MeSH terms

Biofilms
Bioreactors
Denitrification
Microbial Consortia
Nitrification
Nitrogen / metabolism
Waste Disposal, Fluid* / methods
Wastewater*

Substances

Wastewater
Nitrogen