LIP5, a MVB biogenesis regulator, is required for rice growth

Mengxue Wang; Shuwei Luo; Baofang Fan; Cheng Zhu; Zhixiang Chen

doi:10.3389/fpls.2023.1103028

LIP5, a MVB biogenesis regulator, is required for rice growth

Front Plant Sci. 2023 Jan 17:14:1103028. doi: 10.3389/fpls.2023.1103028. eCollection 2023.

Authors

Mengxue Wang¹, Shuwei Luo¹, Baofang Fan², Cheng Zhu¹, Zhixiang Chen^{1

2}

Affiliations

¹ College of Life Science and Key Laboratory of Marine Food Quality and Hazard Controlling Technology of Zhejiang Province, China Jiliang University, Hangzhou, China.
² Department of Botany and Plant Pathology and Center for Plant Biology, Purdue University, West Lafayette, IN, United States.

Abstract

LYST-INTERACTING PROTEIN5 (LIP5) is a conserved regulator of multivesicular body (MVB) biogenesis in eukaryotes. In Arabidopsis, AtLIP5 is a target of stress-responsive MITOGEN-ACTIVATED PROTEIN KINASE3 and 6 and mediates stress-induced MVB biogenesis to promote stress responses. However, Arabidopsis atlip5 knockout mutants are normal in growth and development. Here we report that rice OsLIP5 gene could fully restore both the disease resistance and salt tolerance of the Arabidopsis oslip5 mutant plants to the wild-type levels. Unlike Arabidopsis atlip5 mutants, rice oslip5 mutants were severely stunted, developed necrotic lesions and all died before flowering. Unlike in Arabidopsis, LIP5 regulated endocytosis under both stress and normal conditions in rice. These findings indicate that there is strong evolutionary divergence among different plants in the role of the conserved LIP5-regulated MVB pathway in normal plant growth.

Keywords: LYST-INTERACTING PROTEIN5; SKD1; cellular homeostasis; endocytosis; multivesicular bodies; plant growth; rice.

Grants and funding

This work is supported by China National Major Research and Development Plan (grant no. 0111900), National Natural Science Foundation of China (grant no. 32000143) and Zhejiang Provincial Natural Science Foundation of China (grant no. LQ20C020002) at China Jiliang University.