Transcriptome analysis of growth and shell color between two genetic variants of Corbicula fluminea with different shell colors

Xuefeng Lv; Congdi Wu; Honghui Hu; Yuanshuai Fu; Ling Yan; Zhiyan Wang; Gaolun Lv; Guiling Wang; Zhiyi Bai

doi:10.1016/j.cbd.2023.101056

Transcriptome analysis of growth and shell color between two genetic variants of Corbicula fluminea with different shell colors

Comp Biochem Physiol Part D Genomics Proteomics. 2023 Mar:45:101056. doi: 10.1016/j.cbd.2023.101056. Epub 2023 Jan 21.

Authors

Xuefeng Lv¹, Congdi Wu², Honghui Hu², Yuanshuai Fu², Ling Yan², Zhiyan Wang², Gaolun Lv², Guiling Wang², Zhiyi Bai³

Affiliations

¹ Key Laboratory of Genetic Resources for Freshwater Aquaculture and Fisheries, Shanghai Ocean University, Shanghai 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shangha Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquaculture, Shanghai 201306, China. Electronic address: https://twitter.com/1696704001@qq.com.
² Key Laboratory of Genetic Resources for Freshwater Aquaculture and Fisheries, Shanghai Ocean University, Shanghai 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shangha Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquaculture, Shanghai 201306, China.
³ Key Laboratory of Genetic Resources for Freshwater Aquaculture and Fisheries, Shanghai Ocean University, Shanghai 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shangha Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquaculture, Shanghai 201306, China. Electronic address: zybai@shou.edu.cn.

PMID: 36736150
DOI: 10.1016/j.cbd.2023.101056

Abstract

To clarify the molecular mechanism of the black and yellow shell coloration, we performed a transcriptome analysis of whole tissue of Corbicula fluminea in Hongze Lake (Jiangsu Province, China). After assembly, 335,247 unigenes were obtained, and 136,804 unigenes were functionally identified using public databases (NR, GO, KEGG, eggnog, and Swissprot). 1567 differentially expressed genes (DEGs) were detected through pairwise comparisons, of which 941 DEGs were up-regulated and 626 were down-regulated in the black-shelled clam. We compared the DEGs between two clams and identified some coloration-related genes. Notably, the black-shelled clam was larger than the yellow-shelled. We speculated that higher digestion and anabolic ability of black-shelled clam might lead to this phenomenon. In contrast, the yellow-shelled clam appeared to be more sensitive to environmental stress. The metabolic energy of the yellow-shelled clam was depleted to maintain or recover from stress, and provide less energy for growth. In summary, our finding provides a theoretical basis for the molecular mechanism of pigmentation and the difference of somatotype in bivalve, as well as promotes the future breeding of more elite varieties.

Keywords: Corbicula fluminea; Shell color; Transcriptomic; Variants.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Color
Corbicula* / genetics
Gene Expression Profiling
Pigmentation / genetics
Transcriptome