In vitro and in silico cholinesterase inhibitory and antioxidant effects of essential oils and extracts of two new Salvia fruticosa mill. cultivars (Turgut and Uysal) and GC-MS analysis of the essential oils

Int J Environ Health Res. 2024 Feb;34(2):674-686. doi: 10.1080/09603123.2022.2163988. Epub 2023 Feb 5.

Abstract

The EtOH extracts of the leaves of two new cultivars (Uysal-SFU and Turgut-SFT) of Salvia fruticosa Mill. was tested against acetylcholinesterase (IC50: 30.62 ± 3.27 and 32.97 ± 2.33 µg/mL for SFU and SFT, respectively) and butyrylcholinesterase (IC50: 69.91 ± 1.08 µg/mL and 86.55 ± 1.26 µg/mL), respectively, relevant to Alzheimer's disease. The essential oils showed a stumpy inhibition against AChE and no inhibition against BChE. DPPH radical scavenging activity of the extracts (86.70 ± 0.17% and 86.14 ± 1.13% for SFU and SFT, respectively) was stronger than that of quercetin (85.51 ± 0.17%): Their (1.24 ± 0.05 and 1.04 ± 0.16 for SFU and SFT, respectively) ferric-reducing antioxidant power were close to that of the reference (e.g. quercetin, 1.42 ± 0.14). Molecular docking simulations were performed on their major monoterpenes. Our findings revealed that the leaf EtOH extracts of two cultivars are promising inhibitors of both AChE and BChE.

Keywords: Salvia fruticosa; cholinesterase; enzyme inhibition; essential oil; molecular docking.

MeSH terms

  • Acetylcholinesterase
  • Antioxidants / pharmacology
  • Butyrylcholinesterase
  • Cholinesterase Inhibitors / pharmacology
  • Gas Chromatography-Mass Spectrometry
  • Molecular Docking Simulation
  • Oils, Volatile* / pharmacology
  • Plant Extracts / pharmacology
  • Quercetin
  • Salvia*

Substances

  • Butyrylcholinesterase
  • Antioxidants
  • Acetylcholinesterase
  • Oils, Volatile
  • Quercetin
  • Cholinesterase Inhibitors
  • Plant Extracts