Priming with caffeic acid enhances the potential and survival ability of human adipose-derived stem cells to counteract hypoxia

Abstract

The therapeutic effectiveness of stem cells after transplantation is hampered by the hypoxic milieu of chronic wounds. Prior research has established antioxidant priming as a thorough plan to improve stem cell performance. The purpose of this study was to ascertain how caffeic acid (CA) priming affected the ability of human adipose-derived stem cells (hASCs) to function under hypoxic stress. In order to study the cytoprotective properties of CA, hASCs were primed with CA in CoCl₂ hypoxic conditions. Microscopy was used to assess cell morphology, while XTT, Trypan Blue, X-gal, LDH, Live Dead, scratch wound healing, and ROS assays were used to analyze viability, senescence, cell death, proliferation, and reactive oxygen species prevalence in the cells. According to our findings, CA priming enhances hASCs' ability to survive and regenerate in a hypoxic microenvironment more effectively than untreated hASCs. Our in-vitro research suggested that pre-treatment with CA of hASCs could be a unique way to enhance their therapeutic efficacy and ability to survive in hypoxic microenvironments.

Keywords: Antioxidants; Caffeic acid; Caffeic acid, (CA); Cobalt chloride; Human adipose-derived stem cells; Human adipose-derived stem cells, (hASCs); Hypoxia stress; Mesenchymal stem cells, (MSCs); Pre-conditioning; Reactive oxygen species, (ROS); Stromal vascular fraction, (SVF); oxidative stress, (OS).