Ligustilide induces apoptosis and reduces proliferation in human bladder cancer cells by NFκB1 and mitochondria pathway

Liqi Yin; Lu Ying; Rui Guo; Mingxuan Hao; Youfeng Liang; Ying Bi; Yuan Chen; Changyuan Yu; Zhao Yang

doi:10.1111/cbdd.14207

Ligustilide induces apoptosis and reduces proliferation in human bladder cancer cells by NFκB1 and mitochondria pathway

Chem Biol Drug Des. 2023 Jun;101(6):1252-1261. doi: 10.1111/cbdd.14207. Epub 2023 Feb 15.

Authors

Liqi Yin¹, Lu Ying^{1

2}, Rui Guo¹, Mingxuan Hao¹, Youfeng Liang¹, Ying Bi¹, Yuan Chen³, Changyuan Yu¹, Zhao Yang^{1

2}

Affiliations

¹ Innovation Center of Molecular Diagnostics, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China.
² Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Corps, College of Life Science and Technology, Tarim University, Alar, China.
³ Department of Urology, Dazhou Central Hospital, Dazhou, China.

PMID: 36751909
DOI: 10.1111/cbdd.14207

Abstract

Ligustilide (LIG), the bioactive constituent of Angelica sinensis, may exert potential benefits in cancer treatment. However, the potential mechanism of LIG in the suppression of bladder cancer (BC) has not been reported yet. This study uncovered the inhibitory effect of LIG on the proliferation and cell cycle arrest of BC cells (T24 and EJ-1) along with unveiling the underlying molecular mechanism. The IC₅₀ values of LIG-treated T24 for 24 and 48 h are 39.91 μg/mL (209.8 μM) and 40.94 μg/mL (215.2 μM) separately. The same conditions, the IC₅₀ values of EJ-1 are 45.73 μg/mL (240.4 μM) and 43.81 μg/mL (230.3 μM), separately. Additionally, LIG induced apoptosis and cycle arrest of T24 and EJ-1 cells in sub-G1 phase. Further studies showed that LIG induced apoptosis of BC cells by upregulating Caspase-8, truncated BID (tBID) and BAX proteins, and downregulating NFκB1 (p50) protein. In conclusion, LIG significantly inhibits the growth of BC cells in vitro and in vivo by inducing apoptosis and is inexpensive, making it a promising candidate for novel anti-BC drugs.

Keywords: NFκB1; apoptosis; bladder cancer; ligustilide; proliferation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Apoptosis*
Cell Line, Tumor
Cell Proliferation
Humans
Mitochondria / metabolism
Urinary Bladder Neoplasms* / drug therapy
Urinary Bladder Neoplasms* / metabolism

Substances

ligustilide