Eightfold induction of nicotine elimination in perfused rat liver by pretreatment with phenobarbital

Biochem Biophys Res Commun. 1987 Oct 14;148(1):192-8. doi: 10.1016/0006-291x(87)91094-1.


Elimination of nicotine by isolated rat livers was increased eightfold after pretreatment with phenobarbital (PB) as an inducer of cytochrome P-450 while it was only marginally influenced after pretreatment with 5,6-benzoflavone (BF) as an inducer of cytochrome P-448. Initial rates of cotinine formation were enhanced in the same order of magnitude in PB-induced livers. The 14C-nicotine-derived radioactivity excreted into bile within 2 h ranged between 6 -17% of the dose with only 2.7 fold higher values after PB pretreatment compared to controls.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Benzoflavones / pharmacology
  • Cytochrome P-450 CYP1A2
  • Cytochrome P-450 Enzyme System / biosynthesis*
  • Cytochromes / biosynthesis
  • Enzyme Induction
  • Kinetics
  • Liver / drug effects
  • Liver / metabolism*
  • Male
  • Nicotine / metabolism*
  • Phenobarbital / pharmacology*
  • Rats
  • Rats, Inbred Strains
  • beta-Naphthoflavone


  • Benzoflavones
  • Cytochromes
  • beta-Naphthoflavone
  • Nicotine
  • Cytochrome P-450 Enzyme System
  • Cyp1a2 protein, rat
  • Cytochrome P-450 CYP1A2
  • Phenobarbital