Deep learning-assisted smartphone-based portable and visual ratiometric fluorescence device integrated intelligent gel label for agro-food freshness detection

Zhiwei Lu; Mengjiao Li; Maoting Chen; Qirui Wang; Chun Wu; Mengmeng Sun; Gehong Su; Xianxiang Wang; Yanying Wang; Xinguang Zhou; Jianshan Ye; Tao Liu; Hanbing Rao

doi:10.1016/j.foodchem.2023.135640

Deep learning-assisted smartphone-based portable and visual ratiometric fluorescence device integrated intelligent gel label for agro-food freshness detection

Food Chem. 2023 Jul 1:413:135640. doi: 10.1016/j.foodchem.2023.135640. Epub 2023 Feb 4.

Authors

Zhiwei Lu¹, Mengjiao Li², Maoting Chen², Qirui Wang³, Chun Wu², Mengmeng Sun², Gehong Su², Xianxiang Wang², Yanying Wang², Xinguang Zhou⁴, Jianshan Ye⁵, Tao Liu⁶, Hanbing Rao⁷

Affiliations

¹ College of Science, Sichuan Agricultural University, Xin Kang Road, Yucheng District, Ya'an 625014, PR China. Electronic address: zhiweilu@sicau.edu.cn.
² College of Science, Sichuan Agricultural University, Xin Kang Road, Yucheng District, Ya'an 625014, PR China.
³ School of Mechanical Engineering and Electronic Information, China University of Geoscience, Wuhan 430074, PR China.
⁴ Shenzhen NTEK Testing Technology Co., Ltd., Shenzhen 518000, PR China.
⁵ School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, PR China.
⁶ College of Information Engineering, Sichuan Agricultural University, Xin kang Road, Yucheng District, Ya'an 625014, PR China. Electronic address: liutao@sicau.edu.cn.
⁷ College of Science, Sichuan Agricultural University, Xin Kang Road, Yucheng District, Ya'an 625014, PR China. Electronic address: rhb@sicau.edu.cn.

PMID: 36758385
DOI: 10.1016/j.foodchem.2023.135640

Abstract

Here, a smartphone-assisted dual-color ratiometric fluorescence smart gel label-based visual sensing platform was constructed for real-time evaluation of the freshness of agro-food based on the biogenic amines responses. Green-emission fluorescence carbon dots (CDs) coupled with blue-emission fluorescence bimetallic metal-organic framework (Fe/Zr-MOF) obtained dual-color CDs@Fe/Zr-MOF fluorescence nanoprobe acts as the response units. With the increase of SP and HIS content, the green fluorescence of CDs was enhanced, while the blue fluorescence of Fe/Zr-MOF was quenched. Therefore, this dual-color probe achieved a clear fluorescence color response to biogenic amines. The nanoprobe possessed sensitive and color-responsive with the LODs of 0.17 μM for SP and 2.95 μM for HIS in a wide range of 0-937.5 µM, respectively. Besides, these fluorescent nanoprobes were immobilized on the hydrogel carrier, and the intelligent fluorescent hydrogel tag can be obtained after freeze-drying, which realizes the real-time qualitative monitoring of SP and HIS in pork and shrimp samples.

Keywords: Freshness; Histamine; Smart fluorescent tab; Smartphone; Spermine.

MeSH terms

Carbon
Deep Learning*
Fluorescent Dyes
Hydrogels
Limit of Detection
Quantum Dots*
Seafood
Smartphone

Substances

Hydrogels
Fluorescent Dyes
Carbon