Human chromosome-specific repetitive DNA sequences: novel markers for genetic analysis

Chromosoma. 1987;95(6):375-86. doi: 10.1007/BF00333988.

Abstract

Two recombinant DNA clones that are localized to single human chromosomes were isolated from a human repetitive DNA library. Clone pHuR 98, a variant satellite 3 sequence, specifically hybridizes to chromosome position 9qh. Clone pHuR 195, a variant satellite 2 sequence, specifically hybridizes to chromosome position 16qh. These locations were determined by fluorescent in situ hybridization to metaphase chromosomes, and confirmed by DNA hybridizations to human chromosomes sorted by flow cytometry. Pulsed field gel electrophoresis analysis indicated that both sequences exist in the genome as large DNA blocks. In situ hybridization to intact interphase nuclei showed a well-defined, localized organization for both DNA sequences. The ability to tag specific human autosomal chromosomes, both at metaphase and in interphase nuclei, allows novel molecular cytogenetic analyses in numerous basic research and clinical studies.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Cells, Cultured
  • Chromosome Mapping
  • Chromosomes, Human*
  • Cloning, Molecular
  • Humans
  • Karyotyping
  • Male
  • Nucleic Acid Hybridization
  • Repetitive Sequences, Nucleic Acid*
  • Skin / cytology

Associated data

  • GENBANK/M20262
  • GENBANK/M20263