PCR Allele Competitive Extension (PACE)

Daniel von Maydell

doi:10.1007/978-1-0716-3024-2_18

PCR Allele Competitive Extension (PACE)

Methods Mol Biol. 2023:2638:263-271. doi: 10.1007/978-1-0716-3024-2_18.

Author

Daniel von Maydell¹

Affiliation

¹ Julius Kuehn-Institute (JKI), Institute for Breeding Research on Horticultural Crops, Quedlinburg, Germany. daniel.maydell@julius-kuehn.de.

PMID: 36781648
DOI: 10.1007/978-1-0716-3024-2_18

Abstract

PCR allele competitive extension (PACE™) is a marker system to analyze single nucleotide polymorphisms (SNPs). As strongly related technologies like kompetitive allele-specific PCR (KASP™) or Amplifluor^®, PACE is based on a polymerase chain reaction (PCR) with two competing allele-specific primers and an endpoint fluorescent measurement. The system requires sequence information surrounding the SNP of interest and a qPCR machine or a fluorescent plate reader.

Keywords: Codominant marker system; PCR allele competitive extension (PACE); Single nucleotide polymorphisms (SNPs).

MeSH terms

Alleles
Coloring Agents*
DNA Primers / genetics
Genotype
Polymerase Chain Reaction
Polymorphism, Single Nucleotide*

Substances

DNA Primers
Coloring Agents