The Current State of Nanopore Sequencing

Methods Mol Biol. 2023:2632:3-14. doi: 10.1007/978-1-0716-2996-3_1.


Nanopore sensing is a disruptive, revolutionary way in which to sequence nucleic acids, including both native DNA and RNA molecules. First commercialized with the MinIONTM sequencer from Oxford Nanopore TechnologiesTM in 2015, this review article looks at the current state of nanopore sequencing as of June 2022. Covering the unique characteristics of the technology and how it functions, we then go on to look at the ability of the platform to deliver sequencing at all scales-from personal to high-throughput devices-before looking at how the scientific community is applying the technology around the world to answer their biological questions.

Keywords: Applications; Disruptive; Flongle; GridION; MinION; Nanopore; PromethION; Sequencing.

Publication types

  • Review

MeSH terms

  • High-Throughput Nucleotide Sequencing
  • Nanopore Sequencing*
  • Nanopores*
  • Sequence Analysis, DNA