A dataset of definitive endoderm and hepatocyte differentiations from human induced pluripotent stem cells

Sci Data. 2023 Feb 14;10(1):93. doi: 10.1038/s41597-023-02001-9.

Abstract

Hepatocytes are a major parenchymal cell type in the liver and play an essential role in liver function. Hepatocyte-like cells can be differentiated in vitro from induced pluripotent stem cells (iPSCs) via definitive endoderm (DE)-like cells and hepatoblast-like cells. Here, we explored the in vitro differentiation time-course of hepatocyte-like cells. We performed methylome and transcriptome analyses for hepatocyte-like cell differentiation. We also analyzed DE-like cell differentiation by methylome, transcriptome, chromatin accessibility, and GATA6 binding profiles, using finer time-course samples. In this manuscript, we provide a detailed description of the dataset and the technical validations. Our data may be valuable for the analysis of the molecular mechanisms underlying hepatocyte and DE differentiations.

Publication types

  • Dataset

MeSH terms

  • Cell Differentiation*
  • Endoderm
  • Hepatocytes
  • Humans
  • Induced Pluripotent Stem Cells*
  • Liver