Effective Blocking and Stabilizing Methods Using Synthetic Polymer on ELISA

Tomozumi Noda; Masaru Matsuda; Hirotaka Suzuki; Yusuke Okawa; Motohiro Mitani

doi:10.1007/978-1-0716-2903-1_5

Effective Blocking and Stabilizing Methods Using Synthetic Polymer on ELISA

Methods Mol Biol. 2023:2612:59-71. doi: 10.1007/978-1-0716-2903-1_5.

Authors

Tomozumi Noda¹, Masaru Matsuda¹, Hirotaka Suzuki¹, Yusuke Okawa¹, Motohiro Mitani¹

Affiliation

¹ NOF CORPORATION, Life Science Products Division, Tokyo, Japan.

PMID: 36795359
DOI: 10.1007/978-1-0716-2903-1_5

Abstract

In ELISA, blocking reagents and stabilizers are important to improve the sensitivity and/or quantitative nature of the measurement system. Usually, biological substances such as bovine serum albumin and casein are used, but they still have problems such as lot-to-lot differences and biohazard. Here, we describe the methods using a chemically synthesized polymer, BIOLIPIDURE®, as a new blocking agent and stabilizer that can solve these problems.

Keywords: Antibody; Blocking; Chemically synthesized materials; ELISA; Stabilizer.

MeSH terms

Caseins* / analysis
Enzyme-Linked Immunosorbent Assay / methods
Polymers*
Serum Albumin, Bovine

Substances

Polymers
Caseins
Serum Albumin, Bovine