Anticholinergic effect of resveratrol with vitamin E on scopolamine-induced Alzheimer's disease in rats: Mechanistic approach to prevent inflammation

Ahmed I Foudah; Sushma Devi; Aftab Alam; Mohammad Ayman Salkini; Samir A Ross

doi:10.3389/fphar.2023.1115721

Anticholinergic effect of resveratrol with vitamin E on scopolamine-induced Alzheimer's disease in rats: Mechanistic approach to prevent inflammation

Front Pharmacol. 2023 Feb 2:14:1115721. doi: 10.3389/fphar.2023.1115721. eCollection 2023.

Authors

Ahmed I Foudah¹, Sushma Devi², Aftab Alam¹, Mohammad Ayman Salkini¹, Samir A Ross^{3

4}

Affiliations

¹ Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia.
² Chitkara College of Pharmacy, Chitkara University, Rajpura, Punjab, India.
³ National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS, United States.
⁴ Department of Biomolecular Sciences, School of Pharmacy, The University of Mississippi, University, MS, United States.

Abstract

The most common form of dementia, Alzheimer's disease (AD), is characterized by gradual declines in cognitive abilities and behavior. It is caused by a combination of factors, including amyloid-β (Aβ) accumulation, acetylcholine (ACh) loss, oxidative stress, and inflammation. Phenolic compounds have a variety of health benefits, including antioxidant activities. Thus, the purpose of this study was to investigate how resveratrol (RES) alone and in combination with vitamin E affected rats with AD using scopolamine (SCO). Animals are categorized into groups; (i) control, (ii) SCO (1 mg/kg i.p.), (iii) SCO + donepezil, (iv) SCO + RES (50 mg/kg, p.o.), (v) SCO + RES (75 mg/kg, p.o.), (vi) SCO + RES (50 mg/kg + vitamin E 1 mg/kg, p.o.) for 17 days. In rats, studied behavioural (NOR and EPM) and biochemical characteristics. In addition, brain histopathology was examined to investigate any damage to the hippocampus and neuroprotection. SCO-induced changes in acetylcholinesterase, protein carbonyl, and TNF-α improved after resveratrol treatment. RES increased antioxidant levels, decreased SCO-induced lipid peroxidation, and reversed SCO-mediated changes compared with the drug donepezil. The results indicated that RES and vitamin E had nootropic action in the NOR and EPM tests, measured by the recognition index and the inflection ratio. This study supports the efficacy of RES as a preventive and treatment agent for AD. Vitamin E showed a synergistic effect on RES, which helps in managing cognitive impairment AD.

Keywords: Alzheimer’s disease; dementia; herbal therapies; neurological disorder; resveratrol; vitamin E.

Grants and funding

The authors extend their appreciation to the Deputyship for Resea and Innovation, Ministry of Education in Saudi Arabia for funding this research work through the project number (IF2/PSAU/2022/03/22664).