Effect of NH4+ ions on phi 29 DNA-protein p3 replication: formation of a complex between the terminal protein and the DNA polymerase

J Virol. 1987 Dec;61(12):3983-91. doi: 10.1128/JVI.61.12.3983-3991.1987.


Ammonium ions stimulated the formation of the phi diameter 29 protein p3-dAMP initiation complex by decreasing the Km value for dATP in a purified system containing the viral terminal protein p3, the viral DNA polymerase p2, and the phi 29 DNA-protein p3 complex as a template. In addition, NH4+ ions stimulated the amount of p3-dAMP complex elongation and increased by about twofold the rate of elongation. The stimulatory effect of NH4+ ions on in vitro phi 29 DNA replication is probably related to the formation of a stable complex between the terminal protein and the DNA polymerase, which was detected only in the presence of NH4+ ions.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Ammonium Sulfate / pharmacology*
  • Bacteriophages / drug effects
  • Bacteriophages / genetics*
  • Bacteriophages / physiology
  • DNA Replication / drug effects*
  • DNA, Viral / metabolism
  • DNA-Directed DNA Polymerase / metabolism*
  • Deoxyadenine Nucleotides / metabolism
  • Kinetics
  • Templates, Genetic
  • Viral Proteins / metabolism*
  • Virus Replication / drug effects


  • DNA, Viral
  • Deoxyadenine Nucleotides
  • Viral Proteins
  • DNA-Directed DNA Polymerase
  • Ammonium Sulfate