Isolation and Visualization of Gliding Motility Machinery in Bacteroidota

Satoshi Shibata; Daisuke Nakane

doi:10.1007/978-1-0716-3060-0_21

Isolation and Visualization of Gliding Motility Machinery in Bacteroidota

Methods Mol Biol. 2023:2646:267-276. doi: 10.1007/978-1-0716-3060-0_21.

Authors

Satoshi Shibata¹, Daisuke Nakane²

Affiliations

¹ Division of Bacteriology, Department of Microbiology and Immunology, Faculty of Medicine, Tottori University, Tottori, Japan. sshibata@tottori-u.ac.jp.
² Department of Engineering Science, Graduate School of Informatics and Engineering, The University of Electro-Communications, Tokyo, Japan.

PMID: 36842121
DOI: 10.1007/978-1-0716-3060-0_21

Abstract

Many members of the phylum Bacteroidota (formerly called Bacteroidetes) adhere to and move on solid surfaces. This type of bacterial motility is called gliding and does not involve the conventional bacterial motility machinery, such as flagella and pili. To understand the mechanism of gliding motility of some Bacteroidota bacteria such as a soil bacterium Flavobacterium johnsoniae and a marine bacterium Saprospira grandis, the gliding motility machines of these two bacteria have been analyzed by electron microscopy with negative staining. Here, we describe methods to directly observe the gliding motility machinery in Bacteroidota by transmission electron microscopy.

Keywords: Gliding motility; Negative staining; Osmotically shocked cell; Phylum Bacteroidota; Transmission electron microscopy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins*
Bacteroidetes
Fimbriae, Bacterial
Flavobacterium*

Substances

Bacterial Proteins