Optimized protocol for live imaging of overnutrition-elicited interactions between immune cells and β cells in zebrafish

Bingyuan Yang; Yue Zhang; Liu Yang; Wenbiao Chen

doi:10.1016/j.xpro.2022.102039

Optimized protocol for live imaging of overnutrition-elicited interactions between immune cells and β cells in zebrafish

STAR Protoc. 2023 Mar 17;4(1):102039. doi: 10.1016/j.xpro.2022.102039. Epub 2023 Jan 17.

Authors

Bingyuan Yang¹, Yue Zhang¹, Liu Yang², Wenbiao Chen³

Affiliations

¹ Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.
² Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232, USA; Department of Endocrinology and Metabolism, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, No. 301 Middle Yanchang Road, Shanghai 200072, China.
³ Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232, USA. Electronic address: wenbiao.chen@vanderbilt.edu.

Abstract

Here, we provide an optimized protocol to observe the interactions between infiltrating immune cells and islet β cells using live imaging. This protocol is useful for the characterization of cell-cell interactions and for the direct visualization of immune cell migration to the principal pancreatic islet during islet inflammation. We describe the preparation of zebrafish transgenic lines and detail steps for setting up the fish for live confocal imaging. For more details on the use and execution of this protocol, please refer to Yang et al. (2022).¹.

Keywords: Cell Biology; Immunology; Metabolism; Microscopy; Model Organisms.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Animals
Cell Movement
Insulin-Secreting Cells*
Islets of Langerhans*
Overnutrition*
Zebrafish