Targeted delivery of therapeutic agents to the mouse brain using a stereotactic-guided focused ultrasound device

STAR Protoc. 2023 Feb 23;4(1):102132. doi: 10.1016/j.xpro.2023.102132. Online ahead of print.


Existing protocols of focused ultrasound (FUS) combined with microbubble-mediated blood-brain barrier (BBB) opening (FUS-BBBO) in preclinical research require expensive ultrasound equipment and complex operating procedures. We developed a low-cost, easy-to-use, and precise FUS device for small animal models in preclinical research. Here, we provide a detailed protocol for building the FUS transducer, attaching the transducer to a stereotactic frame for precise brain targeting, applying the integrated FUS device to perform FUS-BBBO in mice, and evaluating the FUS-BBBO outcome. For complete details on the use and execution of this protocol, please refer to Hu et al. (2022).1.

Keywords: Biotechnology and bioengineering; Cancer; Health Sciences; Neuroscience; Physics.