Anti-Obesity Potential through Regulation of Carbohydrate Uptake and Gene Expression in Intestinal Epithelial Cells by the Probiotic Lactiplantibacillus plantarum MGEL20154 from Fermented Food

J Microbiol Biotechnol. 2023 May 28;33(5):621-633. doi: 10.4014/jmb.2212.12005. Epub 2023 Feb 6.

Abstract

We investigated the probiotic characteristics and anti-obesity effect of Lactiplantibacillus plantarum MGEL20154, a strain that possesses excellent intestinal adhesion and viability. The in vitro properties, e.g., gastrointestinal (GI) resistance, adhesion, and enzyme activity, demonstrated that MGEL20154 is a potential probiotic candidate. Oral administration of MGEL20154 to diet-induced obese C57BL/6J mice for 8 weeks resulted in a feed efficacy decrease by 44.7% compared to that of the high-fat diet (HFD) group. The reduction rate of weight gain was about 48.5% in the HFD+MGEL20154 group compared to that of the HFD group after 8 weeks, and the epididymal fat pad was also reduced in size by 25.2%. In addition, the upregulation of the zo-1, pparα, and erk2, and downregulation of the nf-κb and glut2 genes in Caco-2 cells by MGEL20154 were observed. Therefore, we propose that the anti-obesity effect of the strain is exerted by inhibiting carbohydrate absorption and regulating gene expression in the intestine.

Keywords: Lactiplantibacillus plantarum MGEL20154; anti-obesity; erk2; glut2; pparα.

MeSH terms

  • Animals
  • Caco-2 Cells
  • Carbohydrates
  • Diet, High-Fat / adverse effects
  • Fermented Foods*
  • Gene Expression
  • Humans
  • Intestines
  • Lactobacillus plantarum* / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Obesity / metabolism
  • Probiotics* / pharmacology

Substances

  • Carbohydrates