Polishing De Novo Nanopore Assemblies of Bacteria and Eukaryotes With FMLRC2

Mol Biol Evol. 2023 Mar 4;40(3):msad048. doi: 10.1093/molbev/msad048.


As the accuracy and throughput of nanopore sequencing improve, it is increasingly common to perform long-read first de novo genome assemblies followed by polishing with accurate short reads. We briefly introduce FMLRC2, the successor to the original FM-index Long Read Corrector (FMLRC), and illustrate its performance as a fast and accurate de novo assembly polisher for both bacterial and eukaryotic genomes.

Keywords: de novo assembly; nanopore; polishing.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Bacteria / genetics
  • Eukaryota* / genetics
  • Genome, Bacterial
  • High-Throughput Nucleotide Sequencing
  • Nanopores*
  • Sequence Analysis, DNA

Associated data

  • Dryad/10.5061/dryad.gtht76hr3