Application potential of chicken DNA chip in domestic pigeon species - Preliminary results

Saudi J Biol Sci. 2023 Mar;30(3):103594. doi: 10.1016/j.sjbs.2023.103594. Epub 2023 Feb 11.

Abstract

Introducing the SNP technology to pigeon breeding will enhance the competitiveness of a sector that produces one of the healthiest and best quality meats. The present study aimed to test the applicability of the Illumina Chicken_50K_CobbCons array on 24 domestic pigeon individuals from the Mirthys hybrids and Racing pigeon breeds. A total of 53,313 SNPs were genotyped. Principal component analysis shows a significant overlap between the two groups. The chip performed poorly in this data set, with a call rate per sample of 0.474 (49%). The low call rate was likely due to an increase in the evolutionary distance. A total of 356 SNPs were retained after a relatively strict quality control. We have demonstrated that it is technically feasible to use a chicken microarray chip on pigeon samples. Presumably, with a larger sample size and by assigning phenotypic data, efficiency would be improved, allowing more thorough analyses, such as genome-wide association studies.

Keywords: DNA chip; Genotyping; Illumina; Racing pigeon; SNP-array; Squab pigeon.