Genome sequence of Leishmania mexicana MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase

Tom Beneke; Ulrich Dobramysl; Carolina Moura Costa Catta-Preta; Jeremy Charles Mottram; Eva Gluenz; Richard J Wheeler

doi:10.12688/wellcomeopenres.18575.2

Genome sequence of Leishmania mexicana MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase

Wellcome Open Res. 2023 Feb 23:7:294. doi: 10.12688/wellcomeopenres.18575.2. eCollection 2022.

Authors

Tom Beneke^{1

2}, Ulrich Dobramysl³, Carolina Moura Costa Catta-Preta⁴, Jeremy Charles Mottram⁴, Eva Gluenz^{1

5}, Richard J Wheeler³

Affiliations

¹ Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.
² Department of Cell and Developmental Biology, Biocentre, University of Würzburg, Würzburg, 97074, Germany.
³ Peter Medawar Building for Pathogen Research, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
⁴ York Biomedical Research Institute, Department of Biology, University of York, York, UK.
⁵ Institute of Cell Biology, University of Bern, Bern, Switzerland.

Abstract

We present the genome sequence of Leishmania mexicana MNYC/BZ/62/M379 modified to express Cas9 and T7 RNA-polymerase, revealing high similarity to the reference genome (MHOM/GT2001/U1103). Through RNAseq-based annotation of coding sequences and untranslated regions, we provide primer sequences for construct and sgRNA template generation for CRISPR-assisted gene deletion and endogenous tagging.

Keywords: Genome; Leishmania mexicana; Pilon polish.

Grants and funding

200807/Z/16/Z/WT_/Wellcome Trust/United Kingdom