Assay of tryptophan decarboxylase from Catharanthus roseus plant cell cultures by high-performance liquid chromatography

Anal Biochem. 1987 Aug 15;165(1):133-6. doi: 10.1016/0003-2697(87)90210-7.

Abstract

An assay is described for the enzyme tryptophan decarboxylase from plant cell suspension cultures. It is based on the fluorometric detection of tryptamine by HPLC on a LiChrosorb RP-8 Select B column. Tryptophan decarboxylase from Catharanthus roseus was induced by transferring 14-day-old cells into an induction medium. Optimum activity was found 2 days after transfer, the increase being 5- to 10-fold. When kept at -15 degrees C the crude enzyme lost half its activity in about 7 days. The rate of the decarboxylation reaction was linear for at least 3 h at 35 degrees C.

MeSH terms

  • Aromatic-L-Amino-Acid Decarboxylases / analysis*
  • Cells, Cultured
  • Chromatography, High Pressure Liquid
  • Plants, Medicinal / enzymology*
  • Spectrometry, Fluorescence
  • Tryptamines

Substances

  • Tryptamines
  • Aromatic-L-Amino-Acid Decarboxylases