Microinjected glutathione S-transferase Yb subunits translocate to the cell nucleus

Biochem J. 1987 Oct 1;247(1):109-12. doi: 10.1042/bj2470109.

Abstract

We have previously shown that a 30 kDa DNA-binding protein isolated from rat cell nuclei exhibits the chemical and immunological properties of glutathione S-transferase Yb subunits [Bennett, Spector & Yeoman (1986) J. Cell Biol. 102, 600-609]. It was of interest, therefore, to determine whether Yb subunits isolated from rat liver nuclei would return to nuclear fractions upon reintroduction to cell cytoplasms via red-blood-cell-mediated fusion. Labelled Yb subunits were associated with nuclear fractions 60 min after cell fusion. The microinjected protein remained associated with the nuclei for 18 h and was not extractable with low-salt washes. In addition, injected Yb subunits were found to equally distribute between extractable (56%) and residual (44%) nuclear fractions. These experiments demonstrate that glutathione S-transferase Yb subunits isolated from nuclei rapidly translocate to nuclei upon reintroduction into cell cytoplasms.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Nucleus / enzymology*
  • Cells, Cultured
  • Glutathione Transferase / metabolism*
  • Isoenzymes / metabolism*
  • Microinjections
  • Rats
  • Subcellular Fractions / enzymology

Substances

  • Isoenzymes
  • Glutathione Transferase