Rapid biomechanical imaging at low irradiation level via dual line-scanning Brillouin microscopy

Jitao Zhang; Milos Nikolic; Kandice Tanner; Giuliano Scarcelli

doi:10.1038/s41592-023-01816-z

Rapid biomechanical imaging at low irradiation level via dual line-scanning Brillouin microscopy

Nat Methods. 2023 May;20(5):677-681. doi: 10.1038/s41592-023-01816-z. Epub 2023 Mar 9.

Authors

Jitao Zhang^#^{1

2}, Milos Nikolic^#^{3

4

5}, Kandice Tanner⁵, Giuliano Scarcelli^{6

7}

Affiliations

¹ Fischell Department of Bioengineering, University of Maryland, College Park, MD, USA. zhang4@wayne.edu.
² Department of Biomedical Engineering, Wayne State University, Detroit, MI, USA. zhang4@wayne.edu.
³ Fischell Department of Bioengineering, University of Maryland, College Park, MD, USA.
⁴ Maryland Biophysics Program, University of Maryland, College Park, MD, USA.
⁵ Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
⁶ Fischell Department of Bioengineering, University of Maryland, College Park, MD, USA. scarc@umd.edu.
⁷ Maryland Biophysics Program, University of Maryland, College Park, MD, USA. scarc@umd.edu.

^# Contributed equally.

Abstract

Brillouin microscopy is a technique for mechanical characterization of biological material without contact at high three-dimensional resolution. Here, we introduce dual line-scanning Brillouin microscopy (dLSBM), which improves acquisition speed and reduces irradiation dose by more than one order of magnitude with selective illumination and single-shot analysis of hundreds of points along the incident beam axis. Using tumor spheroids, we demonstrate the ability to capture the sample response to rapid mechanical perturbations as well as the spatially resolved evolution of the mechanical properties in growing spheroids.

Rapid biomechanical imaging at low irradiation level via dual line-scanning Brillouin microscopy

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