Genome-guided approaches and evaluation of the strategies to influence bioprocessing assisted morphological engineering of Streptomyces cell factories

Khushboo; Mony Thakur; Punit Kumar; Deepanshi Rajput; Vinod Yadav; Namrata Dhaka; Rishikesh Shukla; Kashyap Kumar Dubey

doi:10.1016/j.biortech.2023.128836

Genome-guided approaches and evaluation of the strategies to influence bioprocessing assisted morphological engineering of Streptomyces cell factories

Bioresour Technol. 2023 May:376:128836. doi: 10.1016/j.biortech.2023.128836. Epub 2023 Mar 9.

Authors

Khushboo¹, Mony Thakur², Punit Kumar³, Deepanshi Rajput⁴, Vinod Yadav², Namrata Dhaka¹, Rishikesh Shukla⁵, Kashyap Kumar Dubey⁶

Affiliations

¹ Department of Biotechnology, Central University of Haryana, Mahendergarh 123031, India.
² Department of Microbiology, Central University of Haryana, Mahendergarh 123031, India.
³ Department of Morphology and Physiology, Karaganda Medical University, Karaganda 100008 Kazakhstan.
⁴ Bioprocess Engineering Laboratory, School of Biotechnology, Jawaharlal Nehru University, New Delhi 110067, India.
⁵ Department of Biotechnology, Institute of Applied Sciences and Humanities, GLA University, Mathura- 281406, U.P., India.
⁶ Bioprocess Engineering Laboratory, School of Biotechnology, Jawaharlal Nehru University, New Delhi 110067, India. Electronic address: kashyapdubey@gmail.com.

PMID: 36898554
DOI: 10.1016/j.biortech.2023.128836

Abstract

Streptomyces genera serve as adaptable cell factories for secondary metabolites with various and distinctive chemical structures that are relevant to the pharmaceutical industry. Streptomyces' complex life cycle necessitated a variety of tactics to enhance metabolite production. Identification of metabolic pathways, secondary metabolite clusters, and their controls have all been accomplished using genomic methods. Besides this, bioprocess parameters were also optimized for the regulation of morphology. Kinase families were identified as key checkpoints in the metabolic manipulation (DivIVA, Scy, FilP, matAB, and AfsK) and morphology engineering of Streptomyces. This review illustrates the role of different physiological variables during fermentation in the bioeconomy coupled with genome-based molecular characterization of biomolecules responsible for secondary metabolite production at different developmental stages of the Streptomyces life cycle.

Keywords: Anti-obesity drug; Bioprocess engineering; Microparticle enhanced cultivation; Morphology engineering; Pellet modulation.

Publication types

Review

MeSH terms

Genomics
Metabolic Networks and Pathways
Streptomyces* / genetics
Streptomyces* / metabolism