Induction of Apoptosis and Decrease of Autophagy in Colon Cancer Cells by an Extract of Lyophilized Mango Pulp

Gustavo Argenor Lozano-Casabianca; Sandra Sulay Arango-Varela; María Elena Maldonado-Celis

doi:10.3390/ijerph20054165

Induction of Apoptosis and Decrease of Autophagy in Colon Cancer Cells by an Extract of Lyophilized Mango Pulp

Int J Environ Res Public Health. 2023 Feb 25;20(5):4165. doi: 10.3390/ijerph20054165.

Authors

Gustavo Argenor Lozano-Casabianca¹, Sandra Sulay Arango-Varela², María Elena Maldonado-Celis¹

Affiliations

¹ School of Nutrition and Dietetics, University of Antioquia, Medellín 050010, Colombia.
² Facultad de Ciencias Exactas y Aplicadas, Instituto Tecnológico Metropolitano (ITM), Institución Universitaria, Medellín 050034, Colombia.

Abstract

Previous studies have indicated that mango fruit has a chemopreventive capacity against colorectal cancer cells. The objective of this research was to evaluate the effect of an aqueous extract of lyophilized mango pulp (LMPE) on colon adenocarcinoma cells (SW480) and their metastatic derivatives (SW620) death and cellular invasion. DNA fragmentation was assessed by TUNEL assay; autophagy and expression of DR4 and Bcl-2 by flow cytometry; the expression of 35 apoptosis-related proteins and of matrix metalloproteinases 7 and 9 by immunodetection; and the invasive capacity of the cells by Boyden chamber. The results showed that LMPE at 30 mg/mL and 48 h of exposure results in DNA fragmentation and apoptosis in SW480 (p < 0.001) and SW620 (p < 0.01) cells. Additionally, LMPE decreased autophagy in the SW480 and SW620 cell lines (p < 0.001), which could sensitize them to the DNA damage generated by LMPE. The LMPE did not modulate the expression of matrix metalloproteinases 7 and 9, nor did it affect cellular invasion processes in the SW480 and SW620 cell lines. In conclusion, LMPE induces apoptosis and decreases autophagy in SW480 and SW620 cells.

Keywords: Mangifera indica L.; apoptosis; autophagy; colorectal cancer; matrix metalloproteinases.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenocarcinoma* / pathology
Apoptosis
Apoptosis Regulatory Proteins / metabolism
Autophagy
Cell Line, Tumor
Colonic Neoplasms*
Humans
Mangifera*
Matrix Metalloproteinases

Substances

Apoptosis Regulatory Proteins
Matrix Metalloproteinases

Grants and funding

This research was funded by the Ministry of Science, Technology, and Innovation; the Ministry of National Education; the Ministry of Commerce, Industry and Tourism; and ICETEX, Colombia, through the Scientific Ecosystem component of the Colombia Científica program (Alianza NanoBioCancer code FP44842-211-2018, Project No. 58580).