A Novel Hyaluronic Acid Matrix Ingredient with Regenerative, Anti-Aging and Antioxidant Capacity

Int J Mol Sci. 2023 Mar 1;24(5):4774. doi: 10.3390/ijms24054774.


Hyaluronic acid (HA) and proteoglycans (such as dermatan sulphate (DS) and chondroitin sulphate (CS)) are the main components of the extracellular matrix of the skin, along with collagen and elastin. These components decrease with age, which implies a loss of skin moisture causing wrinkles, sagging and aging. Currently, the external and internal administration of effective ingredients that can reach the epidermis and dermis is the main alternative for combating skin aging. The objective of this work was to extract, characterise and evaluate the potential of an HA matrix ingredient to support anti-aging. The HA matrix was isolated and purified from rooster comb and characterised physicochemically and molecularly. In addition, its regenerative, anti-aging and antioxidant potential and intestinal absorption were evaluated. The results show that the HA matrix is composed of 67% HA, with an average molecular weight of 1.3 MDa; 12% sulphated glycosaminoglycans, including DS and CS; 17% protein, including collagen (10.4%); and water. The in vitro evaluation of the HA matrix's biological activity showed regenerative properties in both fibroblasts and keratinocytes, as well as moisturising, anti-aging and antioxidant effects. Furthermore, the results suggest that the HA matrix could be absorbed in the intestine, implying a potential oral as well as topical use for skin care, either as an ingredient in a nutraceutical or a cosmetic product.

Keywords: anti-aging; antioxidant; collagen; glycosaminoglycans; hyaluronic acid; skin.

MeSH terms

  • Animals
  • Antioxidants* / metabolism
  • Chickens
  • Chondroitin Sulfates / metabolism
  • Collagen / metabolism
  • Fibroblasts / metabolism
  • Glycosaminoglycans / metabolism
  • Hyaluronic Acid* / metabolism
  • Male
  • Regeneration*
  • Skin Aging* / physiology
  • Skin* / metabolism


  • Antioxidants
  • Chondroitin Sulfates
  • Collagen
  • Glycosaminoglycans
  • Hyaluronic Acid

Grants and funding

Bioiberica S.A.U. provided the study product, as well as the funding for this study.