Anti-inflammatory effects of Peucedanum japonicum Thunberg leaves extract in Lipopolysaccharide-stimulated RAW264.7 cells

J Ethnopharmacol. 2023 Jun 12:309:116362. doi: 10.1016/j.jep.2023.116362. Epub 2023 Mar 10.


Ethnopharmacological relevance: Peucedanum japonicum Thunberg are perennial herbaceous plants known to be cultivated for food and traditional medicinal purposes. P. japonicum has been used in traditional medicine to soothe coughs and colds, and to treat many other inflammatory diseases. However, there are no studies on the anti-inflammatory effects of the leaves.

Aim of the study: Inflammation plays an important role in our body as a defense response of biological tissues to certain stimuli. However, the excessive inflammatory response can lead to various diseases. This study aimed to investigate the anti-inflammatory effects of P. japonicum leaves extract (PJLE) in LPS-stimulated RAW 264.7 cells.

Material and methods: Nitric Oxide (NO) production assay measured by NO assay. Inducible NO synthase (iNOS), COX-2, MAPKs, AKT, NF-κB, HO-1, Nrf-2 were examined by western blotting. PGE2, TNF-α, and IL-6 were analyzed by ELSIA. Nuclear translocation of NF-κB was detected by immunofluorescence staining.

Results: PJLE suppressed inducible nitric oxygen synthase (iNOS) and prostaglandin-endoperoxide synthase 2 (cyclooxygenase-2, COX-2) expression, increased heme oxygenase 1 (HO-1) expression, and decreased nitric oxide production. And PJLE inhibited the phosphorylation of AKT, MAPK, and NF-κB. Taken together, PJLE down-regulated inflammatory factors such as iNOS and COX-2 by inhibiting the phosphorylation of AKT, MAPK, and NF-κB.

Conclusions: These results suggest that PJLE can be used as a therapeutic material to modulate inflammatory diseases.

Keywords: AKT pathway; Anti-inflammatory effects; MAP kinase Signaling system; NF-kappa B; Peucedanum japonicum Thunberg.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / pharmacology
  • Anti-Inflammatory Agents / therapeutic use
  • Cyclooxygenase 2 / metabolism
  • Lipopolysaccharides* / pharmacology
  • Mice
  • NF-kappa B* / metabolism
  • Nitric Oxide / metabolism
  • Nitric Oxide Synthase Type II / metabolism
  • Plant Extracts / pharmacology
  • Proto-Oncogene Proteins c-akt / metabolism
  • RAW 264.7 Cells


  • NF-kappa B
  • Lipopolysaccharides
  • Anti-Inflammatory Agents
  • Proto-Oncogene Proteins c-akt
  • Cyclooxygenase 2
  • Nitric Oxide
  • Plant Extracts
  • Nitric Oxide Synthase Type II