The clinicopathological features of ganglioglioma with CD34 expression and BRAF mutation in patients with epilepsy

Ming-Guo Xie; Xiong-Fei Wang; Jiao Qiao; Jian Zhou; Yu-Guang Guan; Tian-Fu Li; Xue-Ling Qi; Guo-Ming Luan

doi:10.3389/fnmol.2023.1022364

The clinicopathological features of ganglioglioma with CD34 expression and BRAF mutation in patients with epilepsy

Front Mol Neurosci. 2023 Feb 23:16:1022364. doi: 10.3389/fnmol.2023.1022364. eCollection 2023.

Authors

Ming-Guo Xie^{1

2}, Xiong-Fei Wang^{1

2}, Jiao Qiao^{1

2}, Jian Zhou^{1

2}, Yu-Guang Guan^{1

2}, Tian-Fu Li^{2

3

4}, Xue-Ling Qi⁵, Guo-Ming Luan^{1

2

3}

Affiliations

¹ Department of Neurosurgery, Epilepsy Center, Sanbo Brain Hospital, Capital Medical University, Beijing, China.
² Beijing Key Laboratory of Epilepsy, Sanbo Brain Hospital, Capital Medical University, Beijing, China.
³ Beijing Institute for Brain Disorders, Capital Medical University, Beijing, China.
⁴ Department of Neurology, Epilepsy Center, Sanbo Brain Hospital, Capital Medical University, Beijing, China.
⁵ Department of Neuropathology, Epilepsy Center, Sanbo Brain Hospital, Capital Medical University, Beijing, China.

Abstract

Objective: The aim of the study was to evaluate the clinicopathological features, as well as the surgical prognosis, of epilepsy-associated gangliogliomas (GG) with CD34 expression and BRAF^V600E mutation.

Methods: Clinical data of patients who underwent epilepsy surgery for GG were retrospectively studied. Univariate and multivariate analyses were performed to evaluate the correlations of clinical and pathological factors with molecular markers of CD34 expression and BRAF^V600E mutation in GG.

Results: A total of 208 patients with GG had immunohistochemical detection of CD34 expression (positive/negative: 184/24), and among them, 89 patients had immunohistochemical detection of BRAF^V600E mutation (positive/negative: 54/35). By univariate and multivariate analyses, seizure aura (p = 0.025), concordance of ictal electroencephalogram (EEG) findings (p = 0.045) and medial temporal tumor (p = 0.030) were found to be related to CD34 expression, but only hospitalization time (p = 0.042) was different for BRAF-mutated status. In addition, drug-resistant epilepsy (p = 0.040) and concordance of interictal EEG findings (p = 0.009) were found to be associated with tumor progression-free survival (PFS) in univariate analysis, but only concordance of interictal EEG findings was with significance in multivariate analysis. However, CD34 expression or BRAF^V600E mutation in GG was not found to be associated with surgical outcomes of seizure control and tumor PFS.

Conclusion: The CD34 expression or BRAF^V600E mutation in GG may partly influence the distribution of clinicopathological features of patients with epilepsy, but they may be not able to predict the surgical prognosis of seizure outcome and tumor recurrence.

Keywords: BRAF; CD34; Ganglioglioma; epilepsy; surgery.