Structure of the capsular polysaccharide of Haemophilus pleuropneumoniae serotype 5

Eur J Biochem. 1987 Dec 30;170(1-2):185-92. doi: 10.1111/j.1432-1033.1987.tb13685.x.

Abstract

The capsular polysaccharide of Haemophilus (Actinobacillus) pleuropneumoniae serotype 5 (ATCC 33377) was found to be a linear type polysaccharide of a repeating disaccharide unit composed of 2-acetamido-2-deoxy-D-glucose and 3-deoxy-D-manno-2-octulosonic acid (dOclA). By composition analysis, methylation, partial hydrolysis and 1H and 13C nuclear magnetic resonance studies, it was concluded that the capsular polysaccharide is a high-molecular-mass unbranched polymer having the structure: [6)-alpha-D-GlcNAcp-(1-5)-beta-dOclAp-(2]n.

MeSH terms

  • Carbohydrates / analysis
  • Chromatography, Gas
  • Chromatography, Gel
  • Chromatography, Ion Exchange
  • Chromatography, Paper
  • Gas Chromatography-Mass Spectrometry
  • Haemophilus / immunology*
  • Magnetic Resonance Spectroscopy
  • Methylation
  • Polysaccharides, Bacterial* / isolation & purification
  • Serotyping

Substances

  • Carbohydrates
  • Polysaccharides, Bacterial