Pseudotyped viruses have been constructed for many viruses. They can mimic the authentic virus and have many advantages compared to authentic viruses. Thus, they have been widely used as a surrogate of authentic virus for viral function analysis, detection of neutralizing antibodies, screening viral entry inhibitors, and others. This chapter reviewed the progress in the field of pseudotyped viruses in general, including the definition and the advantages of pseudotyped viruses, their potential usage, different strategies or vectors used for the construction of pseudotyped viruses, and factors that affect the construction of pseudotyped viruses.
Keywords: Envelope protein; Inhibitor; Neutralizing antibody; Self-assembled pseudotyped virus; Vector; Virus entry.
© 2023. The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.