Pseudotyped Viruses for Mammarenavirus

Adv Exp Med Biol. 2023;1407:279-297. doi: 10.1007/978-981-99-0113-5_15.


Mammarenaviruses are classified into New World arenaviruses (NW) and Old World arenaviruses (OW). The OW arenaviruses include the first discovered mammarenavirus-lymphocytic choriomeningitis virus (LCMV) and the highly lethal Lassa virus (LASV). Mammarenaviruses are transmitted to human by rodents, resulting in severe acute infections and hemorrhagic fever. Pseudotyped viruses have been widely used as a tool in the study of mammarenaviruses. HIV-1, SIV, FIV-based lentiviral vectors, VSV-based vectors, MLV-based vectors, and reverse genetic approaches have been applied in the construction of pseudotyped mammarenaviruses. Pseudotyped mammarenaviruses are commonly used in receptor research, neutralizing antibody detection, inhibitor screening, viral virulence studies, functional analysis of N-linked glycans, and studies of viral infection, endocytosis, and fusion mechanisms.

Keywords: Arenaviruses; Lassa virus; Lentiviral vectors; Lymphocytic choriomeningitis virus; MLV-based vectors; Mammarenavirus; Pseudotyped viruses; VSV-based vectors.

MeSH terms

  • Arenaviridae* / genetics
  • Arenaviruses, New World* / genetics
  • Humans
  • Lassa virus / genetics
  • Lymphocytic choriomeningitis virus / genetics
  • Viral Pseudotyping