Generating Aedes aegypti Mutant Strains with Transgenic Cas9

Ruichen Sun; Robyn Raban; Omar S Akbari

doi:10.1101/pdb.prot108085

Generating Aedes aegypti Mutant Strains with Transgenic Cas9

Cold Spring Harb Protoc. 2023 Sep 1;2023(9):671-678. doi: 10.1101/pdb.prot108085.

Authors

Ruichen Sun¹, Robyn Raban¹, Omar S Akbari²

Affiliations

¹ Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, California 92093, USA.
² Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, California 92093, USA oakbari@ucsd.edu.

PMID: 36931733
DOI: 10.1101/pdb.prot108085

Abstract

Here, we provide a protocol for generating Aedes aegypti mutant strains via end-joining (EJ) or homology-directed repair (HDR) mechanisms using genetically encoded Cas9.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural

MeSH terms

Aedes* / genetics
Animals
Animals, Genetically Modified
CRISPR-Cas Systems*
Recombinational DNA Repair

Grants and funding

R01 AI151004/AI/NIAID NIH HHS/United States