X-ray inhibits FUT4-mediated proliferation in A549 cells by downregulating SP1 expression

J Pak Med Assoc. 2023 Mar;73(3):494-499. doi: 10.47391/JPMA.5312.


Objective: To identify the mechanism of down-regulation of Lewis Y antigen caused by X-ray irradiation.

Methods: The present original research study was conducted at Zhejiang University City College, Hangzhou, Republic of China, from 2020 to 2022. Western blotting, Co-immunoprecipitation (CO-IP), electrophoretic mobility shift assay and Cell Counting Kit-8 (CCK8) were performed to confirm the effect of X-ray irradiation on A549 cell proliferation and its mechanism. Data was analysed using Statistical Package for Social Sciences (SPSS) 11.5.

Results: The expressions of fucosyltransferase IV and Lewis Y were decreased after X-ray irradiation, thus inhibiting the proliferation of A549 lung cancer cells. Deoxyribonucleic acid damage caused by the irradiation caused higher level of poly- adenosinediphosphate-ribosylated Specific Protein 1(SP1), and translocation of SP1 from the nucleus, decreasing the expression of fucosyltransferase IV and Lewis Y.

Conclusion: There was a significant role of glycosylation in radiation therapy for lung cancer.

Keywords: Fucosyltransferase 4, SP1, X-ray, Lung cancer, Cell proliferation..

MeSH terms

  • A549 Cells
  • Cell Line, Tumor
  • Cell Proliferation
  • Fucosyltransferases* / genetics
  • Fucosyltransferases* / metabolism
  • Humans
  • Lewis Blood Group Antigens* / genetics
  • Lewis Blood Group Antigens* / metabolism
  • Lung Neoplasms*
  • Sp1 Transcription Factor* / genetics
  • Sp1 Transcription Factor* / metabolism
  • X-Rays*


  • Fucosyltransferases
  • FUT4 protein, human
  • Sp1 protein, human
  • Sp1 Transcription Factor
  • Lewis Y antigen
  • Lewis Blood Group Antigens