New insights into the retinal microstructure-diurnal activity relationship in the African five-lined skink (Trachylepis quinquetaeniata) (Lichtenstein, 1823)

Neveen E R El-Bakary; Mohamed A M Alsafy; Samir A A El-Gendy; Samar M Ez Elarab

doi:10.1186/s40851-023-00205-w

New insights into the retinal microstructure-diurnal activity relationship in the African five-lined skink (Trachylepis quinquetaeniata) (Lichtenstein, 1823)

Zoological Lett. 2023 Mar 18;9(1):7. doi: 10.1186/s40851-023-00205-w.

Authors

Neveen E R El-Bakary¹, Mohamed A M Alsafy², Samir A A El-Gendy³, Samar M Ez Elarab⁴

Affiliations

¹ Department of Zoology, Faculty of Science, Damietta University, Damietta, Egypt.
² Anatomy and Embryology Department, Faculty of Veterinary Medicine, Alexandria University, Abees 10Th, Alexandria, Egypt. mohamed.alsafy@alexu.edu.eg.
³ Anatomy and Embryology Department, Faculty of Veterinary Medicine, Alexandria University, Abees 10Th, Alexandria, Egypt.
⁴ Histology and Cytology Department, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt.

Abstract

Background: The retinae of diurnal vertebrates have characteristics. Most lizards are strictly diurnal, and their retinal morphology is still unknown.

Materials and methods: The retina of the African five-lined skink (Trachylepis quinquetaeniata) was studied using light and transmission electron microscopy.

Results: The retina's ten layers were all detected. The inner nuclear layer was the thickest by an average of 67.66 μm, and the inner plexiform layer was 57.564 μm. There were elliptical, long cylindrical, and spherical melanosomes (small and large) in the pigment epithelial layer of the retina. The cylindrical melanosomes had a large area on the lateral surfaces of cones to increase light scatter absorption. The photoreceptor layer of the retina had cones only. There were single and double cones, with the double cones consisting of principal and accessory cones. The cones had inner and outer segments separated by oil droplets. A spherical paraboloid body existed between the limiting membrane and the ellipsoid. All single cones had a paraboloid, and double cones had a large paraboloid in the accessory cone. The presence of paraboloids and large ellipsoids with mitochondria of varying sizes may have helped focus the light on cone segments.

Conclusion: The African five-lined skink's eye was light-adapted due to a variety of retinal specializations related to the demands of its diurnal lifestyle in its environment.

Keywords: Diurnal; Photoreceptors; Reptilian; Retina; Ultrastructure.