Illuminating cellular and extracellular vesicle-mediated communication via a split-Nanoluc reporter in vitro and in vivo

Thomas S van Solinge; Shadi Mahjoum; Stefano Ughetto; Alessandro Sammarco; Marike L D Broekman; Xandra O Breakefield; Killian P O'Brien

doi:10.1016/j.crmeth.2023.100412

Illuminating cellular and extracellular vesicle-mediated communication via a split-Nanoluc reporter in vitro and in vivo

Cell Rep Methods. 2023 Feb 21;3(2):100412. doi: 10.1016/j.crmeth.2023.100412. eCollection 2023 Feb 27.

Authors

Thomas S van Solinge^{1

2}, Shadi Mahjoum¹, Stefano Ughetto^{1

3}, Alessandro Sammarco^{1

4

5}, Marike L D Broekman^{1

2

6}, Xandra O Breakefield¹, Killian P O'Brien¹

Affiliations

¹ Molecular Neurogenetics Unit, Department of Neurology and Center for Molecular Imaging Research, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA.
² Department of Neurosurgery, Leiden University Medical Center, Leiden, the Netherlands.
³ Department of Oncology, University of Turin, Candiolo, Italy.
⁴ Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA, USA.
⁵ Department of Comparative Biomedicine and Food Science, University of Padua, Legnaro, Italy.
⁶ Department of Neurosurgery, Haaglanden Medical Center, The Hague, the Netherlands.

Abstract

Tools to effectively demonstrate and quantify functional delivery in cellular communication have been lacking. This study reports the use of a fluorescently labeled split Nanoluc reporter system to demonstrate and quantify functional transfer between cells in vitro and in a subcutaneous tumor mouse model. Our construct allows monitoring of direct, indirect, and specifically extracellular vesicle-mediated functional communication.

Keywords: cancer; cellular communication; extracellular vesicles; functional transfer.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Communication
Communication
Extracellular Vesicles* / genetics
Luciferases / genetics
Mice

Substances

nanoluc
Luciferases

Abstract

Publication types

MeSH terms

Substances

Grants and funding