Pharmacokinetic study of Strongylocentrotus nudus egg polysaccharide in rats and beagles using a 3H-labeling method

Han Xing; Xiaojie Zhu; Jianmin Liao; Ying Kong; Yayuan Lu; Di Zhao; Ning Li; Xijing Chen; Zhiying Qin

doi:10.3389/fphar.2023.1109084

Pharmacokinetic study of Strongylocentrotus nudus egg polysaccharide in rats and beagles using a ³H-labeling method

Front Pharmacol. 2023 Mar 2:14:1109084. doi: 10.3389/fphar.2023.1109084. eCollection 2023.

Authors

Han Xing^{1

2

3}, Xiaojie Zhu⁴, Jianmin Liao⁴, Ying Kong⁴, Yayuan Lu⁴, Di Zhao⁴, Ning Li⁴, Xijing Chen⁴, Zhiying Qin^{1

2

3}

Affiliations

¹ Department of Pharmacy, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
² Henan Key Laboratory of Precision Clinical Pharmacy, Zhengzhou University, Zhengzhou, China.
³ Henan Engineering Research Center for Application and Translation of Precision Clinical Pharmacy, Zhengzhou University, Zhengzhou, China.
⁴ Clinical Pharmacokinetics Laboratory, China Pharmaceutical University, Nanjing, Jiangsu Province, China.

Abstract

Strongylocentrotus nudus egg polysaccharide (SEP) extracted from sea urchins has potential anticancer activity. However, little is known about its pharmacokinetic properties. To investigate the pharmacokinetics of SEP, it was radiolabeled with tritium. Furthermore, a sensitive, selective, and rapid liquid scintillation counter (LSC) method for quantifying ³H-SEP in biological matrix was validated. The lower quantification limit of the method was 4 Bq. The relative standard deviations (RSDs) of the intra- and inter-day precision were <3.0% and <3.9%, respectively. ³H-SEP was successfully applied to investigate the pharmacokinetics of SEP after intravenous administration of 20, 40, and 80 mg/kg (40 μCi/kg) in rats and 5, 10, and 20 mg/kg (6 μCi/kg) in beagles. The AUC_(0-t) of SEP at three different doses was 487.81 ± 39.99 mg/L*h, 1,003.10 ± 95.94 mg/L*h, and 2,188.84 ± 137.73 mg/L*h in rats and 144.12 ± 3.78 mg/L*h, 322.62 ± 28.03 mg/L*h, and 754.17 ± 37.79 mg/L*h in beagles. The terminal elimination half-life (t_1/2) of SEP was longer in beagles (204.29 ± 139.34 h) than in rats (35.48 ± 6.04 h). The concentration of SEP in plasma declined rapidly in both rats and beagles. All the study results provide detailed pharmacokinetic profiles of SEP in two kinds of animals, which will be helpful for further development.

Keywords: 3H-labeling method; Strongylocentrotus nudus egg polysaccharide; beagles; pharmacokinetics; rats; tritium.

Grants and funding

This study was supported by the Foundation of the He’nan Educational Committee (21A350014) and the Medical Science and Technology Research Program of Henan Province (LHGJ20200284 and LHGJ20220420).