Determination of thiocyanate in plasma and saliva without deproteinisation and its validation as a smoking parameter

J Clin Chem Clin Biochem. 1987 Oct;25(10):711-7. doi: 10.1515/cclm.1987.25.10.711.

Abstract

A simple photometric procedure was developed for the determination of thiocyanate (SCN-) in plasma and saliva without deproteinisation or dialysis. Fe(III) ions form a red coloured complex with SCN- with a maximum absorbance at 460 nm. Mercury(II) nitrate is used to run a sample blank. A manual and an automated version (COBAS BIO) of the method is described. The method is linear up to 5000 mumol/l SCN-. The CV of the between-run precision is 2.8-8% for the manual and 2.6-6.6% for the automated method. The SCN- plasma concentration was 21-134 mumol/l in nonsmokers and 44-260 mumol/l in smokers. In mixed saliva, the concentration is much higher than in plasma: 1.57-5.5 mumol/l in smokers and 0.79-3.9 mumol/l in nonsmokers. Plasma, but not oral fluid SCN-, is a valuable parameter for studying smoking habits in population surveys. However, its use is limited. In our experience only heavy smokers can be distinguished from nonsmokers.

MeSH terms

  • Female
  • Humans
  • Indicators and Reagents
  • Male
  • Parotid Gland / metabolism
  • Photometry
  • Saliva / analysis*
  • Smoking / metabolism*
  • Spectrophotometry, Infrared
  • Spectrophotometry, Ultraviolet
  • Thiocyanates / analysis*
  • Thiocyanates / blood

Substances

  • Indicators and Reagents
  • Thiocyanates